GW 501516

GW-501516 (20mg) is a specialized research reagent utilized primarily to investigate cellular energy metabolism, mitochondrial biogenesis, and the activation of the Peroxisome Proliferator-Activated Receptor delta (PPAR$\delta$) pathway. As a high-affinity selective agonist, it serves as a fundamental tool for exploring the transition from glucose-based to lipid-based energy utilization in various tissue models. Dragon Pharma provides GW-501516 (Cardarine) at a purity level of 99%+, specifically for use in in-vitro laboratory experiments and preclinical animal models.

Overview

In the realm of metabolic biochemistry and exercise physiology, GW-501516 has become a primary instrument for investigators exploring the biological "exercise mimetic" signaling axis. Known scientifically as a PPAR$\delta$ agonist, this compound offers a unique mechanism for studying the upregulation of oxidative muscle fibers and enhanced fatty acid oxidation without the immediate requirement of physical exertion. Dragon Pharma provides GW-501516 20mg at a minimum purity of 99%, ensuring that researchers have access to a high-grade Research Compound capable of producing reproducible and accurate data in complex metabolic models.

As scientific inquiry into Type 2 Diabetes, dyslipidemia, and cardiovascular resilience expands, the need for high-selectivity metabolic modulators like GW-501516 has grown. This expanded profile examines the biochemical characteristics, structural integrity, and multifaceted research applications of GW-501516 20mg.

Molecular Characteristics and Chemical Profile

The structural integrity of GW-501516 allows it to bind to the ligand-binding domain of the PPAR$\delta$ receptor with over 1,000-fold selectivity over other PPAR subtypes. This precision is critical for preventing cross-activation of pathways associated with adipogenesis or inflammatory modulation.

• Chemical Identity: GW-501516 (Cardarine)
• Molecular Formula: $C_{21}H_{18}F_{3}NO_{3}S_{2}$
• Molecular Weight: 453.5 g/mol
• Purity: >99% (HPLC and Mass Spectrometry verified)
• Form: Crystalline powder / Lyophilized powder
• Manufacturer: Dragon Pharma

Research Applications

The compound is utilized in various scientific investigative domains, including:

• Mitochondrial Biogenesis: Mapping the increase in mitochondrial density and ATP production efficiency in skeletal muscle.
• Fatty Acid Oxidation: Investigating the rate of lipid catabolism and the expression of carnitine palmitoyltransferase 1 (CPT1).
• Glucose Homeostasis: Studying the modulation of insulin sensitivity and non-insulin-dependent glucose disposal.
• Muscle Fiber Transformation: Assessing the shift from Type II (glycolytic) to Type I (oxidative) muscle fiber phenotypes.
• Vascular Biology: Evaluating the impact of PPAR$\delta$ activation on endothelial inflammation and atherosclerotic plaque stability.

Pathway and Mechanistic Context

The core value of GW-501516 20mg in a laboratory setting is its ability to manipulate the PPAR$\delta$-PGC-1$\alpha$ Axis independently of actual physical training. In a natural physiological state, PPAR$\delta$ is a major regulator of energy expenditure and fat burning in high-metabolic tissues.

The PPAR$\delta$ Metabolic Axis

Upon activation of PPAR$\delta$ by GW-501516, the cell initiates a global shift in energy management. This promotes the activation of genes responsible for the transport and "burning" of long-chain fatty acids while simultaneously improving systemic metabolic flexibility.

Key Mechanistic Shifts:

• Lipid Catabolism Potentiation: GW-501516 facilitates the oxidation of fats by maintaining high levels of enzymes involved in beta-oxidation, providing a framework for studying obesity resistance.
• Pro-inflammatory Cytokine Inhibition: It modulates the expression of inflammatory markers in the vascular wall, useful for research into chronic systemic inflammation.
• Enhanced Oxidative Capacity: Researchers use GW-501516 to observe how tissues adapt to perceived endurance stress, specifically regarding the increase in oxidative enzyme activity.

Primary Research Applications

1. Metabolic Syndrome and Diabetes Research

GW-501516 is heavily utilized in studies involving insulin resistance and metabolic dysfunction. Statistics indicate that metabolic syndrome affects approximately 34% of adults in the United States. Prevalence varies significantly by racial group; data shows that Non-Hispanic Black women (37%) and Hispanic women (40%) have higher rates compared to Non-Hispanic White women (31%). GW-501516 provides a unique framework for investigating the restoration of metabolic markers in these varied models.

2. Cardiovascular Resilience and Dyslipidemia

Known in research circles for its "lipid-corrective" potential, GW-501516 is used to observe how vascular tissues adapt to high-triglyceride environments. Investigators study changes in HDL (high-density lipoprotein) levels; in preclinical models, Cardarine has shown the ability to increase HDL by up to 79% while reducing LDL and VLDL concentrations.

3. Sarcopenia and Muscle Atrophy Models

In metabolic research, GW-501516 is studied for its ability to maintain muscle oxidative health in models of physical inactivity. Research models focus on how PPAR$\delta$ activation can mitigate the loss of mitochondrial function associated with aging and sedentary lifestyles.

Form and Analytical Testing

At Dragon Pharma, the commitment to scientific accuracy begins with a rigorous manufacturing process. GW-501516 20mg is produced under strict laboratory conditions to ensure the compound remains stable and potent.

• Stability and Purity: The compound is optimized for long-term stability, removing synthesis byproducts that could interfere with lipid-sensing assays.
• Rigorous Analytical Verification: To guarantee a purity level of 99% or higher, every batch undergoes High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) to confirm that the chemical signature matches the verified GW-501516 profile.